Variable stoichiometry of Fe(II)-oxidation in ferritin

Variable stoichiometry of Fe(II)-oxidation in ferritin.

Ferritin is an iron storage protein of wide distribution. The protein, apoferritin, is composed of 24 subunits arranged as a spherical shell with outside diam. 13 nm and inner cavity width -8 nm [I] . Up to 4500 Fe atoms can be stored in this space in the form of a ferric oxhydroxide microcrystalline core. It has been shown that apoferritin catalyses the oxidation of Fe(I1) to Fe(II1) [2-51 and...

FeII induced mineralogical transformations of ferric oxyhydroxides into magnetite of variable stoichiometry and morphology

Iron oxidation chemistry in ferritin. Increasing Fe/O2 stoichiometry during core formation.

The origin of previously observed variations in stoichiometry of iron oxidation during the oxidative deposition of iron in ferritin has been poorly understood. Knowledge of the stoichiometry of Fe(II) oxidation by O2 is essential to establishing the mechanism of iron core formation. In the present work, the amount of Fe(II) oxidized was measured by Mössbauer spectrometry and the O2 consumed by ...

Recombinant ferritin: modulation of subunit stoichiometry in bacterial expression systems.

We describe a strategy for the creation of recombinant ferritin heteropolymers which mimic the natural heterogeneity of this protein. This method entailed the co-expression of cDNA for both ferritin H and ferritin L subunits in a single bacterium using either a bicistronic vector, in which both cDNAs were expressed from the vector, or a dual vector expression strategy, in which each subunit was...

Variable stoichiometry among core ribosomal proteins

1. CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. Understanding the regulation and structure of the eukaryotic ribosome is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribo-som...